Factor VIII Intron 22 Inversion in Severe Hemophilia A Patients in Palestine

Table 1

PCR primer sequences for amplification of factor VIII Inv22.

Primer	Sequence (5′ ⟶ 3′)	Length	GenBank accession no.	Specificity	Product sizes
P	GCC CTG CCT GTC CAT TAC ACT GAT GAC ATT ATG CTG AC	38	AF062514	Forward for int22h1	AB = 10 kb WT, carrier, inversion
Q	GGC CCT ACA ACC ATT CTG CCT TTC ACT TTC AGT GCA ATA	39	X86012	Reverse for int22h1	PQ = 12 kb WT, carrier
A	CAC AAG GGG GAA GAG TGT GAG GGT GTG GGA TAA GAA	36	AF062515	Forward for int22h2 & 3	PB = 11 kb carrier, inversion
B	CCC CAA ACT ATA ACC AGC ACC TTG AAC TTC CCC TCT CAT A	40	AF062516	Reverse for int22h2 & 3	AQ = 11 kb carrier, inversion