Table of Contents
Sequencing
Volume 2011, Article ID 827250, 14 pages
http://dx.doi.org/10.1155/2011/827250
Research Article

Gene Expression in Leaves of Susceptible Glycine max during Infection with Phakopsora pachyrhizi Using Next Generation Sequencing

1Soybean Genomics & Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Beltsville, MD 20705, USA
2Department of Computer and Information Sciences, Towson University, 8000 York Road, Towson, MD 21252, USA
3Crop Genetics Research Unit, USDA-ARS, Stoneville, MS 38776, USA

Received 19 July 2011; Revised 4 November 2011; Accepted 7 November 2011

Academic Editor: Alfredo Ciccodicola

Copyright © 2011 Arianne Tremblay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The Supplementary Material contains tables describing qPCR primer sequences (Supplementary Table 1), the fifty most up and down-regulated genes at 10 dai compare to time-zero (Supplementary Table 2), the complete deep sequencing gene expression data of soybean Williams 82 before infection (time-zero) with soybean rust and 10 dai (Supplementary Table 3), the diversity of proteins containing transcription factor domains encodes by down-regulated and up-regulated genes at 10 dai compared to time-zero (Supplementary Table 4), the expression level of genes involved in salicylic acid, jasmonic acid and ethylene biosynthesis at 10 dai compared to time-zero (Supplementary Table 5), the comparison of gene expression using qPCR and deep sequencing (Supplementary Table 6) and three figures representing the distribution of genes expressed at 10 dai and time-zero (Supplementary Figure 1), and diverse metabolic pathways where genes were differentially regulated at time-zero and 10 dai (Supplementary Figure 2; Steroid biosynthesis, Terpenoid backbone biosynthesis and Brassinosteroid biosynthesis and Supplementary Figure 3; Phenylpropanoid biosynthesis and Flavonoid biosynthesis).

  1. Supplementary Materials