Molecular Cloning, Modeling, and Characterization of Type 2 Metallothionein from Plantago ovata Forsk

Figure 5

Modeling and analysis of PoMT2. Five distinct motifs of PoMT2 are indicated as a, b, c, d, and e and colored black on the 3D model. Hydropathy scores of each amino acid (position 5–77) of PoMT2 are shown at corresponding regions. Multiple sequence alignment (shading showed residues identical for the given position in most sequences) showing relationship between PoMT2 and other plant MTs including Plantago major MT2, Solanum lycopersicum MT2B, Nicotiana glutinosa MT2, Actinidia deliciosa MT2, Malus domestica MT2, Coffea arabica MT1, Arabidopsis thaliana MT2A, Brassica juncea MT22, Brassica rapa subsp. pekinensis MT2, Brassica rapa MT2, Arabidopsis thaliana MT2B, Brassica juncea MT21, Brassica juncea MT25, Brassica juncea MT23, Ricinus communis MT2, Cicer arietinum MT2, Trifolium repens MT2, and Vicia faba MT2 (Uniprot IDs are Q5ZF75, Q40158, Q40396, P43390, O24058, P43396, P25860, P69163, Q39269, P69164, Q38805, P56168, P56172, P56170, P30564, Q39459, P43398, and Q41657, resp.).