Stroke Status Evoked Adhesion Molecule Genetic Alterations in Astrocytes Isolated from Stroke-Prone Spontaneously Hypertensive Rats and the Apigenin Inhibition of Their Expression
Figure 1
A comparison between the gene expression levels of ICAM-1, MCP-1, and VCAM-1 in astrocytes isolated from WKY/IZM and SHRSP/IZM. The astrocytes isolated from WKY/IZM and SHRSP/IZM were washed with FBS-free DMEM, before TNF- was added (a) or not added (b) at 4 hours. Total cellular RNA was isolated from cultured astrocytes. The RNA was transcribed with reverse transcriptase and amplified by RT-PCR. An 18s ribosomal RNA amplicon was used as an internal control for quantitation of the total amount of cDNA. The bars represent the mean ± SD (n = 4). Asterisk (*): compared with the WKY/IZM value. WKY: WKY/IZM; SHRSP: SHRSP/IZM.