Stroke Status Evoked Adhesion Molecule Genetic Alterations in Astrocytes Isolated from Stroke-Prone Spontaneously Hypertensive Rats and the Apigenin Inhibition of Their Expression

Figure 6

The effects of H/R on the expression levels of adhesion molecules in astrocytes isolated from SHRSP and a comparison between the inhibitory effects of apigenin, resveratrol. The astrocytes isolated from SHRSP/IZM was incubated under hypoxia (1% O2, 24 hour) or hypoxia + reoxygenation (21% O2, 3 hour) as previously described [13, 15]. They were incubated for an additional period either before hypoxia or after hypoxia with 50 $\mu$M of apigenin or resveratrol. The ICAM-1, MCP-1, and VCAM-1 mRNA measurements were carried out as described in Figure 1 and in the text. The bars represent the mean ± SD (n = 4). Asterisk (*): $P<.05$ compared with the normal condition values. Nor: normal condition; Hyp: Hypoxia; H/R: hypoxia and reoxygenation.