Lentiviral-Mediated shRNA Silencing of PDE4D Gene Inhibits Platelet-Derived Growth Factor-Induced Proliferation and Migration of Rat Aortic Smooth Muscle Cells

Figure 4

Knockdown of endogenous PDE4D does not affect PDGF-induced cAMP level in VSMC. Bar graph illustrates PDGF-BB induced cAMP level in noninfected cells and cells infected with lentivirus particles carrying either PDE4D or nonsense shRNA. There were no significant differences in cAMP level between cells infected with lentivrius carrying PDE4D shRNA and noninfected cells, cells infected with lentivirus particles carrying nonsense shRNA. Values represent means ± SEM from three separate experiments.*P less than .05 versus control.