Mild Hypothermia Attenuates Intercellular Adhesion Molecule-1 Induction via Activation of Extracellular Signal-Regulated Kinase-1/2 in a Focal Cerebral Ischemia Model
Figure 4
Effect of ERK1/2 inhibition on the STAT3 phosphorylation and DNA binding activity. (a) Western blot analysis demonstrates that STAT3 activation at 2 hours after ischemic insult is increased in normothermic group (37, ). This increase was reduced by hypothermia (33, ). In hypothermic group, U0126 treatment (33, ) enhanced STAT3 phosphorylation to the level of normothermia group (37, ). * versus sham; # versus hypothermic ischemia without U0126. (b) DNA binding activity assay of STAT3 using nuclear fraction from ischemic brain under hypothermia condition shows increased binding activity of STAT3 at 2 hours after MCAO (). STAT3 binding was further augmented by U0126 treatment (). * versus sham; # versus ischemia without U0126.
