Short-Term Bioassay of Chlorophenol Compounds Using <i>Euglena gracilis</i>

Ahmed, Hoda; Häder, Donat-Peter

doi:https://doi.org/10.3814/2010/421950

SRX Ecology

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Research Article | Open Access

Volume 2010 | Article ID 421950 | https://doi.org/10.3814/2010/421950

Short-Term Bioassay of Chlorophenol Compounds Using Euglena gracilis

Hoda Ahmed¹and Donat-Peter Häder¹

Received04 Aug 2009

Revised03 Sept 2009

Accepted22 Sept 2009

Published17 Nov 2009

Abstract

Short-term bioassay of chlorophenol compounds such as di- and pentachlorophenol (DCP and PCP) on the movement parameters and photosynthesis activity is tested using Euglena gracilis. Cells were exposed to different concentrations of 2,4-dichlorophenol (2,4-DCP) and pentachlorophenol (PCP) for different incubation times. Results of acute toxicity tests of cell motility showed that Euglena was highly sensitive to PCP after direct exposure in comparison to 2,4-DCP (EC50 were 26.8 mgL−1 and 205.2 mgL−1), respectively. In contrast, 2,4-dichlorophenol shows significant impact on the movement and photosynthesis of the Euglena after longer incubation times (24 hours). Photosynthetic yield of Euglena was suppressed at concentrations ≥1.5 and ≥12 mgL−1 of pentachlorophenol and 2,4-dichlorophenol, respectively. This proves inactivation of photosystem II through inhibition of both electron transport and potential energy of photosynthetic membrane, whereas lower concentrations disturbed Photosystem II making it work ineffectively. Rapid, sensitive, and automatic measurements of several parameters are the main advantages of the studied bioassay.

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Copyright © 2010 Hoda Ahmed and Donat-Peter Häder. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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