Table of Contents
Scholarly Research Exchange
Volume 2009, Article ID 824949, 10 pages
http://dx.doi.org/10.3814/2009/824949
Research Article

Analysis of PfEMP1—var Gene Sequences in Different Plasmodium falciparum Malarial Parasites

1Molecular Biology Research Laboratory, Department of Zoology and Centre for Advanced Studies, University of Pune, Pune 411007, India
2Malaria Section, National Centre for Cell Science, Pune 411007, India

Received 4 September 2008; Accepted 5 February 2009

Copyright © 2009 Aarti Ozarkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

DBLα primers of var gene were used for amplification of parasite genomic DNA. The primers were specific for the two highly conserved sites in DBLα domain and flank the polymorphic segment of DBLα domain. The amino acid sequences were DIGDIIRG and DYVPQYLR. The genomic DNA from parasites amplifies multiple bands in the size range of 350–700 base pair (bp) when amplified with the primer1 and primer 2.

  1. Supplementary Figures