Thrombosis

Thrombosis / 2012 / Article / Fig 5

Clinical Study

The Role of Dermcidin Isoform 2: A Two-Faceted Atherosclerotic Risk Factor for Coronary Artery Disease and the Effect of Acetyl Salicylic Acid on It

Figure 5

Lineweaver-Burk plot of the inhibition of nitric oxide synthase activated by insulin in the cell-free homogenate from the goat artery endothelial cells. The cell-free homogenate of the endothelial cells from the carotid artery of the goat was prepared as described in Section 2. Lineweaver-Burk plot was constructed by adding different amounts of -arginine to the reaction mixture containing 100 μunits/mL insulin in the presence or absence of 0.1 μM dermcidin. The line A represents the formation of NO in the presence of insulin, and the line B represents the formation of NO in the presence of both the insulin and dermcidin. Each point represents mean of 5 different experiments each in triplicate.
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