TY - JOUR A2 - Klatser, Paul R. AU - Sagonda, Tichaona AU - Mupfumi, Lucy AU - Manzou, Rumbidzai AU - Makamure, Beauty AU - Tshabalala, Mqondisi AU - Gwanzura, Lovemore AU - Mason, Peter AU - Mutetwa, Reggie PY - 2014 DA - 2014/05/20 TI - Prevalence of Extensively Drug Resistant Tuberculosis among Archived Multidrug Resistant Tuberculosis Isolates in Zimbabwe SP - 349141 VL - 2014 AB - We conducted a cross-sectional study of second line drug resistance patterns and genetic diversity of MDR-TB isolates archived at the BRTI-TB Laboratory, Harare, between January 2007 and December 2011. DSTs were performed for second line antituberculosis drugs. XDR-TB strains were defined as MDR-TB strains with resistance to either kanamycin and ofloxacin or capreomycin and ofloxacin. Strain types were identified by spoligotyping. No resistance to any second line drugs was shown in 73% of the isolates, with 23% resistant to one or two drugs but not meeting the definition of XDR-TB. A total of 26 shared types were identified, and 18 (69%) matched preexisting shared types in the current published spoligotype databases. Of the 11 out of 18 clustered SITs, 4 predominant (>6 isolates per shared type) were identified. The most and least abundant types were SIT 1468 (LAM 11-ZWE) with 12 (18%) isolates and SIT 53 (T1) with 6 (9%) isolates, respectively. XDR-TB strains are rare in Zimbabwe, but the high proportion of “pre-XDR-TB” strains and treatment failure cases is of concern. The genetic diversity of the MDR-TB strains showed no significant association between SITs and drug resistance. SN - 2090-150X UR - https://doi.org/10.1155/2014/349141 DO - 10.1155/2014/349141 JF - Tuberculosis Research and Treatment PB - Hindawi Publishing Corporation KW - ER -