Comparison of DNA Extraction Protocols and Molecular Targets to Diagnose Tuberculous Meningitis

Table 6

LoD, efficiency, Ct, and Tm results for real time PCR.

Target	LoD (CFU)	Efficiency	Ct
			Median	Median
IS6110	10^0	1,07	31,09	89,8
Hsp 65 KDa	10^2	1,02	25,78	86,8
MPB64	10^3	1,07	35,07	90,7

LoD: limit of detection; Ct: cycle threshold; : temperature of melting.