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Tuberculosis Research and Treatment
Volume 2017, Article ID 8340746, 6 pages
https://doi.org/10.1155/2017/8340746
Research Article

Phenotypic and Genotypic Analysis of Multidrug-Resistant Mycobacterium tuberculosis Isolates from Sudanese Patients

1Department of Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan
2Microbiology Department, Faculty of Medical Laboratory Sciences, Ibn Sina University, Khartoum, Sudan
3Department of Bioinformatics, Africa City of Technology, Khartoum, Sudan
4Sudan Academy of Sciences, Khartoum, Sudan
5Department of Epidemiology, Tropical Medicine Research Institute, National Center for Research, Khartoum, Sudan
6Department of Microbiology, Faculty of Medical Laboratory Sciences, Omdurman Islamic University, Khartoum, Sudan
7Veterinary Research Institute, Khartoum, Sudan
8Microbiology Department, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan
9National Reference Laboratory-Tuberculosis (NRL-TB), National Public Health Laboratory, Khartoum, Sudan
10Department of Microbiology and Parasitology, College of Medicine, University of Bisha, Bisha, Saudi Arabia

Correspondence should be addressed to Solima M. A. Sabeel; moc.liamg@98amilos

Received 22 July 2016; Revised 7 November 2016; Accepted 22 December 2016; Published 18 January 2017

Academic Editor: Vincent Jarlier

Copyright © 2017 Solima M. A. Sabeel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Currently, mutations in rpoB, KatG, and rrs genes and inhA promoter were considered to be involved in conferring resistance to rifampicin, isoniazid, and streptomycin in Mycobacterium tuberculosis (MTB). Objective. The aims of this study were to detect the prevalence of first-line tuberculosis (TB) drug resistance among a group of previously treated and newly detected TB patients, to determine the association between prevalence of multidrug resistance (MDR) and demographic information (age and sex), to explain genes correlated with MDR Mycobacterium tuberculosis, and to characterize MTB via 16S ribosomal RNA (16S rRNA) analysis. Methods. A hundred MTB isolates from Sudanese pulmonary TB patients were included in the study. The proportional method of drug susceptibility test was carried out on Löwenstein-Jensen media. Multiplex PCR of rpoB and KatG genes and inhA promoter was conducted; then rrs genes were amplified by conventional PCR and were sequenced. The sequences of the PCR product were compared with known rrs gene sequences in the GenBank database by multiple sequence alignment tools. Result. The prevalence of MDR was 14.7% among old cases and 5.3% among newly diagnosed cases. Conclusion. Mutations in rrs could be considered as a diagnostic marker.