The Scientific World Journal

The Scientific World Journal / 2002 / Article

Peer-Reviewed Protocol | Open Access

Volume 2 |Article ID 540109 | 3 pages | https://doi.org/10.1100/tsw.2002.845

Isolation of Peripheral Blood Mononuclear Cells from Macaques on a Density Barrier

Received07 Mar 2002
Revised14 May 2002
Accepted15 May 2002

Abstract

The standard techniques for the isolation of human peripheral blood mononuclear cells (PBMCs) using commercial “lymphocyte isolation media” cannot be satisfactorily extended to experimental animals without manipulating either the density or the osmolality of the medium. PBMCs from Macaques can also be isolated from whole blood by sedimentation on to a density barrier containing approx. 10% iodixanol, polysucrose (Ficoll) with a density of approx 1.074 g/ml.


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