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Volume 2, Pages 1356-1360
Peer-Reviewed Protocol

Formation of Self-Generated Gradients of Iodixanol

School of Biomolecular Sciences, Liverpool John Moores University, UK

Received 7 March 2002; Accepted 27 March 2002

Copyright © 2002 John Graham.


The formation of self-generated gradients of iodixanol from a solution of uniform concentration requires the use of vertical or near-vertical rotors. The density profile that is generated depends upon the sedimentation path length of the rotor, centrifugation time, RCF and temperature. Modulation of the starting concentration changes the density range of the gradient. This Protocol Article illustrates the effect of these parameters on gradient shape in a few selected rotors. Because the gradients are formed by the centrifugal field, they are highly reproducible and easy to execute.