The Scientific World Journal

The Scientific World Journal / 2002 / Article

Peer-Reviewed Protocol | Open Access

Volume 2 |Article ID 695254 | 5 pages | https://doi.org/10.1100/tsw.2002.834

Separation of Membrane Vesicles and Cytosol from Cultured Cells and Bacteria in a Preformed Discontinuous Gradient

Received07 Mar 2002
Revised14 May 2002
Accepted15 May 2002

Abstract

There are many situations when it is necessary to separate rapidly and efficiently a cytosolic and a membrane vesicle fraction from either cultured cells or from bacteria. Flotation of the vesicles through a low-density barrier from a dense sample zone using the low viscosity medium iodixanol allows complete separation of these compartments. As the sample is exposed to the gmax the tendency of the proteins to sediment overcomes any diffusion in the opposite direction.


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