The Scientific World Journal

The Scientific World Journal / 2002 / Article

Peer-Reviewed Protocol | Open Access

Volume 2 |Article ID 849523 | 4 pages | https://doi.org/10.1100/tsw.2002.831

Isolation of Human Polymorphonuclear Leukocytes (Granulocytes) from a Leukocyte-Rich Fraction

Received07 Mar 2002
Revised14 May 2002
Accepted15 May 2002

Abstract

Human peripheral blood polymorphonuclear leukocytes (PMNs) or granulocytes from a leukocyte-rich plasma (LRP) are banded at an interface between two layers of iodixanol. If the denser layer of iodixanol is omitted the PMNs may alternatively be pelleted. The procedure can be adapted to blood from other species by small changes to the density of the two iodixanol layers. The method works optimally with EDTA- or citrate-anticoagulated blood.


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