The Scientific World Journal

The Scientific World Journal / 2002 / Article

Peer-Reviewed Protocol | Open Access

Volume 2 |Article ID 968679 | 4 pages | https://doi.org/10.1100/tsw.2002.836

Purification of Peroxisomes in a Self-Generated Gradient

Received07 Mar 2002
Revised14 May 2002
Accepted15 May 2002

Abstract

In iodixanol, peroxisomes are the densest organelle in the light mitochondrial fraction and are therefore easily separated from the other components (lysosomes, mitochondria, etc.) in a self-generated gradient. Self-generated gradients make sample handling very easy and are highly reproducible but need to be formed in either a vertical, near-vertical, or small volume high-performance fixed-angle rotor. The resolution of the peroxisomes is far superior than that in sucrose and, unlike in PercollⓇ there is no contamination from endoplasmic reticulum.


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