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TheScientificWorldJOURNAL
Volume 4 (2004), Pages 622-627
http://dx.doi.org/10.1100/tsw.2004.122
Research Article

Detection and Characterization of β-Lactam Resistance in Bacillus cereus PTCC 1015

1Pharmaceutical Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IRAN
2Biotechnology Section, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IRAN

Received 18 February 2004; Revised 22 July 2004; Accepted 4 August 2004

Academic Editor: Hans-Peter Schmauder

Copyright © 2004 Javad Behravan and Zeinab Rangsaaz.

Abstract

In the present study, detection, isolation, and characterization of β-lactamases from Bacillus cereus PTCC 1015 were investigated. B. cereus was inoculated in nutrient broth containing ampicillin (50 μg.ml−1) for 24 h (35°C, 200 rpm). Activity measurements were carried out against ampicillin (0.1 mg.ml−1) and cephalexin (0.08 mg.ml−1) by a spectrophotometric method at different conditions (pH 6–10, temperatures 25–45°C).Maximum penicillinase and cephalosporinase activity was observed at pH 7. The optimized temperatures for penicillinase and cephalosporinase activity were 30 and 40°C, respectively. At the above conditions, maximum enzymatic activity was calculated as 0.89 ± 0.014 and 0.037 ± 0.001 units against ampicillin and cephalexin.