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TheScientificWorldJOURNAL
Volume 10, Pages 2003-2018
http://dx.doi.org/10.1100/tsw.2010.195
Peer-Reviewed Protocol

Design and Generation of MLPA Probe Sets for Combined Copy Number and Small-Mutation Analysis of Human Genes: EGFR as an Example

1Laboratory of Cancer Genetics, Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznan, Poland
2Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, USA
3Ludwig Center at Dana-Farber/Harvard Cancer Center, Boston, MA, USA
4Division of Translational Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA

Received 26 July 2010; Revised 6 September 2010; Accepted 23 September 2010

Academic Editor: Steve Brown

Copyright © 2010 Malgorzata Marcinkowska et al.

Citations to this Article [16 citations]

The following is the list of published articles that have cited the current article.

  • Malgorzata Marcinkowska, Maciej Szymanski, Wlodzimierz J. Krzyzosiak, and Piotr Kozlowski, “Copy number variation of microRNA genes in the human genome,” BMC Genomics, vol. 12, 2011. View at Publisher · View at Google Scholar
  • Malgorzata Marcinkowska-Swojak, Barbara Uszczynska, Marek Figlerowicz, and Piotr Kozlowski, “An MLPA-Based Strategy for Discrete CNV Genotyping: CNV-miRNAs as an Example,” Human Mutation, vol. 34, no. 5, pp. 763–773, 2013. View at Publisher · View at Google Scholar
  • Malgorzata Marcinkowska-Swojak, Katarzyna Klonowska, Marek Figlerowicz, and Piotr Kozlowski, “An MLPA-based approach for high-resolution genotyping of disease-related multi-allelic CNVs,” Gene, 2014. View at Publisher · View at Google Scholar
  • S. A. Kravchenko, S. Yu. Chernushyn, A. M. Kucherenko, O. O. Soloviov, and L. A. Livshits, “Development of MLPA approach for SNP detection in MTHFR, F and F2 genes,” Biopolymers and Cell, vol. 31, no. 4, pp. 309–315, 2015. View at Publisher · View at Google Scholar
  • Katarzyna Klonowska, Magdalena Ratajska, Karol Czubak, Alina Kuzniacka, Izabela Brozek, Magdalena Koczkowska, Marcin Sniadecki, Jaroslaw Debniak, Dariusz Wydra, Magdalena Balut, Maciej Stukan, Agnieszka Zmienko, Beata Nowakowska, Irmgard Irminger-Finger, Janusz Limon, and Piotr Kozlowski, “Analysis of large mutations in BARD1 in patients with breast and/or ovarian cancer: the Polish population as an example,” Scientific Reports, vol. 5, pp. 10424, 2015. View at Publisher · View at Google Scholar
  • Wojciech Jozwicki, Janusz Kowalewski, Piotr Kozlowski, Marzena Anna Lewandowska, Karol Czubak, and Katarzyna Klonowska, “The Use of a Two-Tiered Testing Strategy for the Simultaneous Detection of Small EGFR Mutations and EGFR Amplification in Lung Cancer,” Plos One, vol. 10, no. 2, 2015. View at Publisher · View at Google Scholar
  • Malgorzata Marcinkowska-Swojak, Izabela Szczerbal, Hubert Pausch, Joanna Nowacka-Woszuk, Krzysztof Flisikowski, Stanislaw Dzimira, Wojciech Nizanski, Rita Payan-Carreira, Ruedi Fries, Piotr Kozlowski, and Marek Switonski, “Copy number variation in the region harboring SOX9 gene in dogs with testicular/ovotesticular disorder of sex development (78,XX; SRY-negative),” Scientific Reports, vol. 5, pp. 14696, 2015. View at Publisher · View at Google Scholar
  • Karol Czubak, Marzena Anna Lewandowska, Katarzyna Klonowska, Krzysztof Roszkowski, Janusz Kowalewski, Marek Figlerowicz, and Piotr Kozlowski, “High copy number variation of cancer-related microRNA genes and frequent amplification of DICER1 and DROSHA in lung cancer,” Oncotarget, vol. 6, no. 27, pp. 23399–23416, 2015. View at Publisher · View at Google Scholar
  • Lucyna Budzko, Malgorzata Marcinkowska-Swojak, Paulina Jackowiak, Piotr Kozlowski, and Marek Figlerowicz, “Copy number variation of genes involved in the hepatitis C virus-human interactome,” Scientific Reports, vol. 6, pp. 31340, 2016. View at Publisher · View at Google Scholar
  • Malgorzata Marcinkowska-Swojak, Luiza Handschuh, Pawel Wojciechowski, Michal Goralski, Kamil Tomaszewski, Maciej Kazmierczak, Krzysztof Lewandowski, Mieczyslaw Komarnicki, Jacek Blazewicz, Marek Figlerowicz, and Piotr Kozlowski, “A new MLPA-based method for the detection of acute myeloid leukemia-specific NPM1 mutations and simultaneous analysis of gene copy number alterations,” Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 2016. View at Publisher · View at Google Scholar
  • Anna Samelak-Czajka, Maja Szymanska, Agnieszka Zmienko, Piotr Kozlowski, and Marek Figlerowicz, “Arabidopsis thaliana population analysis reveals high plasticity of the genomic region spanning MSH2, AT3G18530 and AT3G18535 genes and provides evidence for NAHR-driven recurrent CNV events occurring in this location,” BMC Genomics, vol. 17, no. 1, 2016. View at Publisher · View at Google Scholar
  • Katarzyna Klonowska, Luiza Handschuh, Aleksandra Swiercz, Marek Figlerowicz, and Piotr Kozlowski, “MTTE: An innovative strategy for the evaluation of targeted/ exome enrichment efficiency,” Oncotarget, vol. 7, no. 41, pp. 67266–67276, 2016. View at Publisher · View at Google Scholar
  • Anna Samelak-Czajka, Malgorzata Marszalek-Zenczak, Malgorzata Marcinkowska-Swojak, Piotr Kozlowski, Marek Figlerowicz, and Agnieszka Zmienko, “MLPA-Based Analysis of Copy Number Variation in Plant Populations,” Frontiers in Plant Science, vol. 8, 2017. View at Publisher · View at Google Scholar
  • Cezary Cybulski, Jan Lubinski, Piotr Kozlowski, Wojciech Kluzniak, Natalia Krawczynska, Karol Czubak, Katarzyna Klonowska, Bogna Rusak, Anna Jakubowska, Magdalena Ratajska, Danuta Vasilevska, and Marzena Wojciechowska, “The 30 kb deletion in the APOBEC3 cluster decreases APOBEC3A and APOBEC3B expression and creates a transcriptionally active hybrid gene but does not associate with breast cancer in the European population,” Oncotarget, vol. 8, no. 44, pp. 76357–76374, 2017. View at Publisher · View at Google Scholar
  • Magdalena Dabrowska, Karol Czubak, Wojciech Juzwa, Wlodzimierz J Krzyzosiak, Marta Olejniczak, and Piotr Kozlowski, “qEva-CRISPR: a method for quantitative evaluation of CRISPR/Cas-mediated genome editing in target and off-target sites,” Nucleic Acids Research, 2018. View at Publisher · View at Google Scholar
  • A. Fernández Asensio, T. Iglesias, A. Cotarelo, M. Espina, E. Blanco-González, L.M. Sierra, and M. Montes-Bayón, “Multiplex polymerase chain reaction in combination with gel electrophoresis-inductively coupled plasma mass spectrometry: A powerful tool for the determination of gene copy number variations and gene expression changes,” Analytica Chimica Acta, 2018. View at Publisher · View at Google Scholar