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TheScientificWorldJOURNAL
Volume 11, Pages 2150-2159
http://dx.doi.org/10.1100/2011/340278
Research Article

Stem Cell Heterogeneity of Mononucleated Cells from Murine Peripheral Blood: Molecular Analysis

1Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 43600 Bangi, Malaysia
2Department of Microbiology, Faculty of Applied Science, Universiti Teknologi MARA, Selangor, 40450 Shah Alam, Malaysia
3Faculty of Dentistry, Universiti Kebangsaan Malaysia, Kuala Lumpur Campus, 50300 Kuala Lumpur, Malaysia

Received 13 September 2011; Accepted 18 October 2011

Academic Editors: A. Favaretto and C. Hosing

Copyright © 2011 Muhammad Dain Yazid et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The main purpose of this paper was to determine the heterogeneity of primary isolated mononucleated cells that originated from the peripheral blood system by observing molecular markers. The isolated cells were cultured in complete medium for 4 to 7 days prior to the separation of different cell types, that is, adherent and suspension. Following a total culture time of 14 days, adherent cells activated the Cd105 gene while suspension cells activated the Sca-1 gene. Both progenitor markers, Cbfa-1 and Ostf-1, were inactivated in both suspension and adherent cells after 14-day culture compared to cells cultured 3 days in designated differentiation medium. In conclusion, molecular analyses showed that primary mononucleated cells are heterogeneous, consisting of hematopoietic stem cells (suspension) and mesenchymal stem cells (adherent) while both cells contained no progenitor cells.