The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils

Figure 2

The bioluminescence signal and optical density (OD) of E. coli-lux (3 × 10⁶ cells) incubated in the presence of various amounts of H₂O₂ (mM) and 2 μg/well MPO in phosphate buffer at 37°C. RLU: (■) 0 mM, (●) MPO + 0 mM, (▲) 2 mM, (▾) MPO + 2 mM, and (♦) 20 mM; OD_620 nm: (□) 0 mM, (○) MPO + 0 mM, (∆) 2 mM, (∇) MPO + 2 mM, and (♢) 20 mM of H₂O₂. Relative luminescence unit (RLU) and OD values are shown as the mean ± SD of measurements from three parallel wells.