The Real-Time-Based Assessment of the Microbial Killing by the Antimicrobial Compounds of Neutrophils
Figure 3
The bioluminescence signal (a) and colony forming units (CFU)/250 μL (b) of E. coli-lux (1 × 106 cells) incubated in the presence of 2.5 × 105 neutrophils and 0.4% serum for opsonization in gHBSS medium at 37°C: (■) bacteria, (●) bacteria + serum, (▲) bacteria + neutrophils and (▾) bacteria + neutrophils + serum. Relative luminescence unit (RLU) values are shown as the mean ± S.D. of measurements from three parallel wells. Arrows point out the onset of the killing.
