Embryonic Regulation of the Mouse Hematopoietic Niche
Figure 5
Hematopoietic environment in fetal liver. (a) (upper) Structure of fetal liver, which primarily consists of hematopoietic cells, sinusoid endothelial cells, and hepatoblasts. (lower) Confocal images of FL expressing Dlk-1/c-Kit (Right). FL sections were prepared from ICR mouse embryos at 12.5 dpc, stained with indicated antibodies, and observed by confocal microscopy. c-Kit (green), Dlk-1 (red), and TOTO-3 (blue). (b) Intrinsic and extrinsic regulation in FL. (upper) Transcription factors (specifically, PU.1, Hoxb3, and Hoxb4) and cytokines (such as SCF and EPO) play important roles in HSC expansion and differentiation in FL. (lower) Liver sections were prepared from ICR mouse embryos at 12.5 dpc and stained with Dlk-1 (green), EPO (red) and TOTO-3 (blue) (Left), and Dlk-1 (green), SCF (red), and TOTO-3 (blue) (Right).
