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The Scientific World Journal
Volume 2012, Article ID 105712, 7 pages
Research Article

Restructuring BOD : COD Ratio of Dairy Milk Industrial Wastewaters in BOD Analysis by Formulating a Specific Microbial Seed

1Environmental Biotechnology Division, Institute of Genomics and Integrative Biology, Delhi 110007, India
2Botany Department, Jamia Hamdard University, Hamdard Nagar, New Delhi 110062, India
3Patent Division, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi 110067, India

Received 30 March 2012; Accepted 5 June 2012

Academic Editors: H. A. Alegria, J. Canário, C. W. Choi, C. García, and D. Kar Chowdhuri

Copyright © 2012 Purnima Dhall et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


BOD (Biochemical oxygen demand) is the pollution index of any water sample. One of the main factors influencing the estimation of BOD is the nature of microorganisms used as seeding material. In order to meet the variation in wastewater characteristics, one has to be specific in choosing the biological component that is the seeding material. The present study deals with the estimation of BOD of dairy wastewater using a specific microbial consortium and compares of the results with seeding material (BODSEED). Bacterial strains were isolated from 5 different sources and were screened by the conventional BOD method. The selected microbial seed comprises of Enterobacter sp., Pseudomonas sp. BOD : COD (Chemical oxygen demand) ratio using the formulated seed comes in the range of 0.7-0.8 whereas that using BODSEED comes in the ratio of 0.5-0.6. The ultimate BOD (UBOD) was also performed by exceeding the 3-day dilution BOD test. After 90 days, it has been observed that the ratio of BOD : COD increased in case of selected consortium 7 up to 0.91 in comparison to 0.74 by BODSEED. The results were analyzed statistically by t-test and it was observed that selected consortium was more significant than the BODSEED.