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The Scientific World Journal
Volume 2012 (2012), Article ID 121253, 6 pages
http://dx.doi.org/10.1100/2012/121253
Research Article

In Vitro Evaluation of Antiprotozoal and Antiviral Activities of Extracts from Argentinean Mikania Species

1Cátedra de Farmacognosia, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 2°P, 1113, Buenos Aires, Argentina
2Cátedra de Inmunología, IDEHU UBA-CONICET, Facultad de Farmacia y Bioquímica y Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Junín 956 4°P, 1113, Buenos Aires, Argentina
3Cátedra de Virología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junín 956 4°P, 1113, Buenos Aires, Argentina
4Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), UBA-CONICET, Junín 956 4°P, 1113, Buenos Aires, Argentina
5INQUINOA-CONICET y Instituto de Química Orgánica, Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 471, T4000INI San Miguel de Tucumán, Argentina

Received 18 May 2012; Accepted 5 June 2012

Academic Editors: Y. Hashiguchi and K. E. Kester

Copyright © 2012 Laura C. Laurella et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the antiprotozoal and antiviral activities of four Argentinean Mikania species. The organic and aqueous extracts of Mikania micrantha, M. parodii, M. periplocifolia, and M. cordifolia were tested on Trypanosoma cruzi epimastigotes, Leishmania braziliensis promastigotes, and dengue virus type 2. The organic extract of M. micrantha was the most active against T. cruzi and L. braziliensis exhibiting a growth inhibition of 7 7 . 6 ± 4 . 5 % and 8 4 . 9 ± 6 . 1 %, respectively, at a concentration of 10 μg/ml. The bioguided fractionation of M. micrantha organic extract led to the identification of two active fractions. The chromatographic profile and infrared analysis of these fractions revealed the presence of sesquiterpene lactones. None of the tested extracts were active against dengue virus type 2.