Simple Reversed-Phase HPLC Method with Spectrophotometric Detection for Measuring Acetaminophen-Protein Adducts in Rat Liver Samples

Table 3

Results of the intraday variability study on the proposed HPLC method based on three different concentrations of APAP-Cys added to distilled water and to blank rat liver matrix protein dispersion^a.

APAP-Cys injected (μg)	Peak area ratio; (mean ± SD, 𝑛=3)	RSD (%)
Samples in distilled water
0.156	0.005 ± 0.002	0.87
0.625	0.020 ± 0.001	0.60
1.250	0.042 ± 0.001	0.61
Samples in rat liver matrix protein dispersion
0.156	0.004 ± 0.001	1.15
0.625	0.017 ± 0.001	1.32
1.250	0.039 ± 0.003	1.05

^aEach sample was analyzed once a day for three consecutive days.