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The Scientific World Journal
Volume 2012, Article ID 147158, 8 pages
Research Article

Characterization of the Immune Response Induced by a Commercially Available Inactivated Bluetongue Virus Serotype 1 Vaccine in Sheep

1VISAVET Health Surveillance Centre and Animal Health Department, Veterinary Faculty, Complutense University of Madrid, Avenida Puerta de Hierro s/n, 28040 Madrid, Spain
2Department of Comparative Pathology, Veterinary Faculty, University of Córdoba, Animal Health Building, Rabanales Campus, 14014 Córdoba, Spain

Received 18 October 2011; Accepted 22 December 2011

Academic Editors: E. Carrillo and K. E. Kester

Copyright © 2012 Ana Cristina Pérez de Diego et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The protective immune response generated by a commercial monovalent inactivated vaccine against bluetongue virus serotype 1 (BTV1) was studied. Five sheep were vaccinated, boost-vaccinated, and then challenged against BTV1 ALG/2006. RT-PCR did not detect viremia at any time during the experiment. Except a temperature increase observed after the initial and boost vaccinations, no clinical signs or lesions were observed. A specific and protective antibody response checked by ELISA was induced after vaccination and boost vaccination. This specific antibody response was associated with a significant increase in B lymphocytes confirmed by flow cytometry, while significant increases were not observed in T lymphocyte subpopulations (CD4+, CD8+, and WC1+), CD25+ regulatory cells, or CD14+ monocytes. After challenge with BTV1, the antibody response was much higher than during the boost vaccination period, and it was associated with a significant increase in B lymphocytes, CD14+ monocytes, CD25+ regulatory cells, and CD8+ cytotoxic T lymphocytes.