Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk
Figure 2
Optimization of TC assay conditions. (a) The concentration of DNA aptamer was () 0.0250 μg mL−1, (∘) 0.0188 μg mL−1, and () 0.0125 μg mL−1 and the concentration of TC-HRP conjugate was 1 μg mL−1 in a solid line and 0.5 μg mL−1 in a dotted line. (b) Effect of incubation temperature on ELAA performance: () room temperature and (∘) 4°C. (c) Influence of thermal denaturation of DNA aptamer: () 95°C, 10 min and (∘) no treatment.