Research Article

Sensitivity and Selectivity on Aptamer-Based Assay: The Determination of Tetracycline Residue in Bovine Milk

Figure 2

Optimization of TC assay conditions. (a) The concentration of DNA aptamer was ( ) 0.0250 μg mL−1, (∘) 0.0188 μg mL−1, and ( ) 0.0125 μg mL−1 and the concentration of TC-HRP conjugate was 1 μg mL−1 in a solid line and 0.5 μg mL−1 in a dotted line. (b) Effect of incubation temperature on ELAA performance: ( ) room temperature and (∘) 4°C. (c) Influence of thermal denaturation of DNA aptamer: ( ) 95°C, 10 min and (∘) no treatment.
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(a)
159456.fig.002b
(b)
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(c)