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The Scientific World Journal
Volume 2012, Article ID 160434, 5 pages
Research Article

A Study on L-Asparaginase of Nocardia levis MK-VL_113

Department of Botany and Microbiology, Acharya Nagarjuna University, Guntur 522 510, India

Received 31 October 2011; Accepted 26 December 2011

Academic Editor: Xavier Thomas

Copyright © 2012 Alapati Kavitha and Muvva Vijayalakshmi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


An enzyme-based drug, L-asparaginase, was produced by Nocardia levis MK-VL_113 isolated from laterite soils of Guntur region. Cultural parameters affecting the production of L-asparaginase by the strain were optimized. Maximal yields of L-asparaginase were recorded from 3-day-old culture grown in modified asparagine-glycerol salts broth with initial pH 7.0 at temperature 3 0 C . Glycerol (2%) and yeast extract (1.5%) served as good carbon and nitrogen sources for L-asparaginase production, respectively. Cell-disrupting agents like EDTA slightly enhanced the productivity of L-asparaginase. Ours is the first paper on the production of L-asparaginase by N. levis.