Research Article

A Study on L-Asparaginase of Nocardia levis MK-VL_113

Table 5

Effect of cell-disrupting agents on the release of L-asparaginase from 72 h old culture of Nocardia levis.

Cell disrupting agents (50 šœ‡ g/ml)Release of L-asparaginase from the culture (U/mg of cell dry wt.)

*Control 5 . 0 6 Ā± 0 . 0 0 2
EDTA 5 . 3 4 Ā± 0 . 0 4
Lysozyme 5 . 2 9 Ā± 0 . 0 1 9
Penicillin-G 5 . 1 1 Ā± 0 . 0 8 2
SDS 4 . 0 1 Ā± 0 . 1 6 3

Values are the means of three replicates Ā± S D . *Cell disruption using homogenizer.