Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples

Table 3

Linearity, range and limit of quantification results obtained with the Milliflex Quantum method: analysis done using data generated for the accuracy testing of the Milliflex Quantum method, comparison between fluorescence and viability results (𝑛=10).

Microorganism	Linearity				Range (CFU)		Limit of Quantification (CFU)
	Fluorescence 𝑅^2-value	Fluorescence linear regression slope	Viability 𝑅^2-value	Viability linear regression slope	Fluorescence	Viability	Fluorescence lowest count mean	Viability lowest count mean	Lowest mean count with compendial method
Candida albican; ATCC 10231	0.98	1.02	0.98	1.01	0 to 163	0 to 161	7	7	7
Aspergillus brasiliensis; ATCC 16404	0.95	1.09	0.96	1.10	0 to 124	0 to 120	10	10	9
Bacillus subtilis ATCC 6633	0.96	1.16	0.96	0.97	0 to 121	0 to 97	6	6	6
Escherichia coli ATCC 8739	0.95	1.06	0.96	1.02	0 to 120	0 to 114	4	4	5

ATCC, American Type Culture Collection; CFU, Colony-forming unit.