Fluorescence-Based Rapid Detection of Microbiological Contaminants in Water Samples

Table 4

Detection of microorganisms in pharmaceutical waters: comparison between fluorescence and viability results obtained with Milliflex Quantum and compendial method results (𝑛=10). “a” and “b” are 2 different types of waters, sampled in the same pharmaceutical plant.

Water	Tested volume	Incubation time	Fluorescence count mean ± SD (CFU)	Fluorescence recovery ± SD (%)	Viability count mean ± SD (CFU)	Viability recovery ± SD (%)	Incubation time	Count mean ± SD (CFU)
1	1 mL	40 h	248.3±31.0	113±19	196.3±21.5	89±14	5 days	248.3±31.0
2	10 mL	24 h	105.4±8.0	110±11	98.8±8.8	103±11	5 days	95.8±6.0
3a	10^−2mL	30 h	21.6±5.0	72±19	22.8±6.4	76±24	7 days	29.9±3.9
3b	10^−2mL	24 h	30.6±2.7	77±15	30.6±1.7	77±14	7 days	39.9±7.1
4a	10^−1mL	24 h	179.7±5.5	95±7	178.3±11.5	95±9	5 days	188.7±12.9
4b	50 mL	30 h	24.0±3.0	74±19	27.7±4.7	86±24	5 days	32.3±7.0
5	1 mL	26 h	21.8±8.5	109±55	17.0±5.1	85±37	5 days	20.0±6.4

CFU, Colony-forming unit; SD, Standard deviation.