Activation of β-Adrenoceptors by Dobutamine May Induce a Higher Expression of Peroxisome Proliferator-Activated Receptors δ (PPARδ) in Neonatal Rat Cardiomyocytes

Figure 3

Effects of calcium-mediated signaling inhibitors on dobutamine-induced actions in neonatal rat cardiomyocytes. The neonatal rat cardiomyocytes were treated with 1 μmol/L cyclosporine A (CsA, calcineurin inhibitor) or 1 μmol/L KN93 (CaMK inhibitor) at 30 minutes prior to incubation with 1 μmol/L dobutamine for 4 hours. The cells were then harvested to measure PPARδ protein expression (a) and cTnI phosphorylation (b) using Western blotting analysis. All values are expressed as mean ± SEM (𝑛=6 per group). **𝑃<0.01 as compared with control cells. ^##𝑃<0.01 as compared to the control cells incubated with dobutamine only.

(a)

(b)