Transcriptome and Proteome Research in Veterinary Science: What Is Possible and What Questions Can Be Asked?

Figure 3

Shotgun proteomics or MudPIT uses multidimensional chromatographic separation to reduce complexity of protein lysates. The selection of the type of chromatographic columns depends on the question asked and the technical equipment. The different protein lysate fractions obtained by chromatographic separation ideally contain only one protein type which is subsequently identified by mass spectrometric analysis and data base search.