Distribution of Extrasynaptic NMDA Receptors on Neurons

Figure 2

Double immunogold labeling of clathrin-coated pits/vesicles (CCP/V; arrowheads) associated with a “bare density” (A; arrow) and extrasynaptic membrane regions (B, C) in the CA1 stratum radiatum of the P2 hippocampus, labeled for NR1 (A, B; 5 nm gold) or NR2A/B (C; 5 nm gold), and clathrin (10 nm gold). The “bare density” (labeled for NR1) on the dendrite in A actually has a fairly close association with an adjacent process. In the dendrite in B, NR1 and clathrin label an early, flat CCP/V adjacent to a CCP/V that is pinching off. In C, NR2A/B labeling on the cell surface is continuous with a clathrin-labeled CCP/V. Scale bar is 100 nm. Reprinted from part of Figure 3 from Petralia et al. [15].