Biomedical Evaluation of Cortisol, Cortisone, and Corticosterone along with Testosterone and Epitestosterone Applying Micellar Electrokinetic Chromatography

Table 1

Analytical extraction efficiency test of the analyzed steroids after various extraction columns and solvents.

Column extraction	Concentration added (ng/mL)	Dichloromethane			Methanol
		Concentration found (ng/mL) (𝑛=3); (mean ± SD)	Recovery (%)	RSD (%)	Concentration found (ng/mL) (𝑛=3); (mean ± SD)	Recovery (%)	RSD (%)
C8	10	5.5 ± 0.7	54.6	12.9	6.2 ± 0.7	62.3	11.6
	50	53.1 ± 6.1	53.1	11.4	63.4 ± 6.5	63.4	10.3
	200	111.4 ± 10.9	55.7	9.8	123.4 ± 12.2	61.7	9.9
C18	10	8.8 ± 0.6	88.4	6.6	9.1 ± 0.6	90.9	6.4
	50	42.5 ± 3.5	85.1	8.2	44.7 ± 3.2	89.4	7.1
	100	81.7 ± 5.9	81.7	7.3	89.8 ± 6.2	89.8	6.9
CN	10	3.4 ± 0.3	33.7	8.8	3.6 ± 0.3	35.6	7.6
	50	15.6 ± 1.7	31.2	10.7	17.1 ± 1.4	34.2	8.2
	100	30.8 ± 2.1	30.8	6.9	36.3 ± 3.5	36.3	9.7
HLB	10	8.9 ± 0.2	89.6	2.2	9.6 ± 0.2	95.8	2.3
	50	45.2 ± 2.4	90.4	5.4	47.3 ± 2.0	94.6	4.2
	100	92.1 ± 4.5	92.1	4.9	46.1 ± 1.8	92.1	3.9