Thyroid Hormone-Induced Cytosol-to-Nuclear Translocation of Rat Liver Nrf2 Is Dependent on Kupffer Cell Functioning
Figure 2
Gadolinium chloride (GdCl3) administration is associated with suppression of T3-induced activation of liver Nrf2 signaling. Determinations were performed at 2 h after T3 administration in rats pretreated with GdCl3 for 72 h. (a) Levels of cytosolic Nrf2 protein (68 kDa), β-actin (43 kDa), α-tubulin (52 kDa), and lamin A/C (65 kDa); (b) levels of nuclear Nrf2 protein, lamin A/C, and α-tubulin; (c) nuclear/cytosolic Nrf2 content ratios and net effects of T3 and GdCl3 treatments (inset); (d) levels of heme oxygenase 1 (HO-1) protein (33 kDa); (e) levels of catalytic subunit of glutamate cysteine ligase (GCLC) protein (73 kDa); (f) levels of thioredoxin (Trx) protein (12 kDa). Values shown represent means ± SEM for 3 to 6 rats per experimental group. Significance (; two-way ANOVA and the Newman-Keuls’ test) is indicated by the letters identifying each experimental group. Significance in the inset of (c) was calculated by Student’s t-test for unpaired data.
