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The Scientific World Journal
Volume 2012, Article ID 308034, 5 pages
Research Article

Antibiotic Synergy Interaction against Multidrug-Resistant Pseudomonas aeruginosa Isolated from an Abattoir Effluent Environment

1Department of Microbiology, Ambrose Alli University, Private Mail Bag 14, Ekpoma 310001, Edo State, Nigeria
2Department of Microbiology and Biotechnology, Western Delta University, Private Mail Bag 10, Oghara 331100, Delta State, Nigeria
3Department of Biochemistry and Microbiology, University of Fort Hare, Private Bag X1314, Alice 5700, South Africa

Received 22 October 2011; Accepted 23 November 2011

Academic Editor: Hendrik K. van Saene

Copyright © 2012 Etinosa O. Igbinosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Pseudomonas aeruginosa is an opportunistic pathogen in environmental waters with a high prevalence of multidrug resistance. In this study the synergistic efficacy of synergy antibiotic combinations in multidrug-resistant P. aeruginosa strains isolated from an abattoir effluent was investigated. Water samples were processed using membrane filtration; Pseudomonas was isolated with Pseudomonas Isolation Agar and confirmed using polymerase chain reaction with specie-specific primer. Susceptibility studies and in vitro synergy interaction testing were carried out, employing agar dilution and Etest procedure, respectively. Resistance was noted for clinically relevant antipseudomonal agents tested. Finding from antibiotic synergy interaction studies revealed that cefepime, imipenem, and meropenem combined with amikacin resulted in statistically significant ( 𝑃 < 0 . 0 0 0 1 ) in vitro antibiotics synergy interaction, indicating the possible use of this regimen in treatment of pseudomonal infections.