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The Scientific World Journal
Volume 2012, Article ID 341764, 5 pages
http://dx.doi.org/10.1100/2012/341764
Research Article

Phytophthora capsici Epidemic Dispersion on Commercial Pepper Fields in Aguascalientes, Mexico

1Instituto Tecnológico El Llano Aguascalientes, Ags., Km 18, Carr. Ags.-S.L.P., 20330, Mexico
2CIRCE-INIFAP, Km 6.5 Carr. Celaya-San Miguel de Allende, 38110 Celaya, Gto., Mexico

Received 1 November 2011; Accepted 18 January 2012

Academic Editor: Sarabjit Mastana

Copyright © 2012 Adrián Zapata-Vázquez et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Chili pepper blight observed on pepper farms from north Aguascalientes was monitored for the presence of Phytophthora capsici during 2008–2010. Initially, ELISA tests were directed to plant samples from greenhouses and rustic nurseries, showing an 86% of positive samples. Later, samples of wilted plants from the farms during the first survey were tested with ELISA. The subsequent survey on soil samples included mycelia isolation and PCR amplification of a 560 bp fragment of ITS-specific DNA sequence of P. capsici. Data was analyzed according to four geographical areas defined by coordinates to ease the dispersal assessment. In general, one-third of the samples from surveyed fields contained P. capsici, inferring that this may be the pathogen responsible of the observed wilt. Nevertheless, only five sites from a total of 92 were consistently negative to P. capsici. The presence of this pathogen was detected through ELISA and confirmed through PCR. The other two-thirds of the negative samples may be attributable to Fusarium and Rhizoctonia, both isolated instead of Phytophthora in these areas. Due to these striking results, this information would be of interest for local plant protection committees and farmers to avoid further dispersal of pathogens to new lands.