A Novel HPLC Method for the Concurrent Analysis and Quantitation of Seven Water-Soluble Vitamins in Biological Fluids (Plasma and Urine): A Validation Study and Application

Table 5

Retention times and peak areas of analysed vitamins. Relative standard deviations (%RSD) of retention times and peak areas for 7 water-soluble vitamins spiked at 20 ng μL⁻¹ in artificial plasma obtained in the analysis for repeatability and reproducibility.

Vitamin	Repeatability^a		Reproducibility^b
	Retention time %RSD	Area %RSD	Retention time %RSD	Area %RSD
C	0.56%	2.23%	1.97%	4.56%
B1	0.99%	3.65%	2.76%	6.89%
B2	0.13%	1.87%	0.89%	3.3%
B5	0.90%	2.25%	2.75%	4.1%
PLP	0.46%	2.22%	1.93%	3.77%
B9	0.18%	2.69%	1.2%	2.98%
B12	0.67%	2.98%	2.37%	6.07%