Table 5: Retention times and peak areas of analysed vitamins. Relative standard deviations (%RSD) of retention times and peak areas for 7 water-soluble vitamins spiked at 20 ng μL−1 in artificial plasma obtained in the analysis for repeatability and reproducibility.

VitaminRepeatabilityaReproducibilityb
Retention time %RSDArea %RSDRetention time %RSDArea %RSD

C0.56%2.23%1.97%4.56%
B10.99%3.65%2.76%6.89%
B20.13%1.87%0.89%3.3%
B50.90%2.25%2.75%4.1%
PLP0.46%2.22%1.93%3.77%
B90.18%2.69%1.2%2.98%
B120.67%2.98%2.37%6.07%