Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling

Figure 4

Application of MSR in identifying double-infection of HPV in a cervical cancer sample. (a) A sample infected with two strains of HPV was selected according to HPV array results. The HPV sequences in the specimen were amplified with a pair of PCR primers specific to the L1 region. (b) Alignment of the L1 sequences of several HPV genotypes. (c) PCR direct sequencing results showed a mixed chromatography trace representing two HPV genotypes. (d) MSR estimated the LRi ratio between the major/minor sequences. (e) The MSR output showed that the sample was infected with HPV genotypes 33 and 81.