Research Article

Mixed Sequence Reader: A Program for Analyzing DNA Sequences with Heterozygous Base Calling

Table 1

Feature comparison between MSR and other currently available DNA sequencing software.

Feature/softwareMSRIndelligentShiftDetectorCHILDPolyScan

Directly reads trace files (abi format)YesNoYesYesNo
Maps sequence to reference databaseYesNoNoNoNo
Detects indelsYesYesYesYesYes
Decomposes 2 mixed sequences from a single traceYesYesNoYesNo
Estimates the ratio of 2 mixed sequences in one traceYesNoNoYesNo
Accepts NGS dataNoNoNoNoYes