Assessment of Tools for Marker-Assisted Selection in a Marine Commercial Species: Significant Association between MSTN-1 Gene Polymorphism and Growth Traits

Table 1

Description of RFLPs in five candidate genes related to growth traits in gilthead seabream analyzed in this work.

Gene	PCR fragment	Enzyme used to detect polymorphism	Polymorphic region	RFLP alleles
GH	A: Exon 1 to intron2	HaeIII & HapII	Intron I	Size (micros.)
GH	B: Exon 4 to 3′UTR	HaeIII, MboII, PstI & TaqI	N.P.	—
IGF-I	A: Promoter to exon 1	TaqI	Promoter	T→G/C
IGF-I	B: Exon 1 to exon 2	HaeIII, EcoRI DraI, HapII & BspLU11I	N.P.	—
MSTN-1	A: Promoter to exon 2	HaeIII	Exon 1	C→T
		DraI	Intron 1	A→C
MSTN-1	B: Exon 2 to exon 3	HaeIII	Intron 2	G→T
PRL	A: Promoter to exon 1	SspBI & DraI	Promoter	Size (micros.)
PRL	B: Exon 1 to exon 2	EcoRI	Intron 1	A→G
		BspLU11I	Intron 1	C→T
PRL	C: Exon 3 to exon 5	HaeIII	Intron 3/Exon 4	Size (indel)
		FokI	Intron 3 & 4/Exon 3	Size (indel)
SL	A: Promoter to exon 2	HaeIII, HapII, AfaI, DraI & TaqI	N.P.	—
SL	B: Exon 4 to exon 5	Tru9I, TaqI, SspI, HaeIII	N.P.	—

N.P.: nonpolymorphic; GH: growth hormone; IGF-1: insulin-like growth factor I; MSTN: myostatin; PRL: prolactin; SL: somatolactin.