Monocytes Do Not Transdifferentiate into Proper Osteoblasts

Table 1

Sequences of used primer pairs and gen bank accession number of target genes.

Gene	Gene bank accession number	Forward primer 5′→3′	Reverse primer 5′→3′	Annealing temperature	Amplicon size [bp]
GAPDH	NM_002046.3	GTC AGT GGT GGA CCT GAC CT	AGG GGT CTA CAT GGC AAC TG	58°C	419 bp
CD34	NM_001025109	AGAAAGGCTGGGCGAAGACCC	AGTGGGGAAGGGTTGGGCGT	56°C	311 bp
Collagen Type 1	NM_000088.3	CAGCCGCTTCACCTACAGC	TTTTGTATTCAATCACTGTCTTGCC	56°C	84 bp
Runx2	NM_001015051.3	TGCCTAGGCGCATTTCAGGTGC	GGTGGTGGTGCATGGCGGAA	58°C	359 bp
Osterix	NM_152860	CCCAGGCAACACTCCTACTC	GGCTGGATTAAGGGGAGCAAA	62°C	175 bp
Osteonectin	NM_003118	AGCACCCCATTGACGGGTA	GGTCACAGGTCTCGAAAAAGC	60°C	105 bp
Osteopontin	NM_000582	CTCCATTGACTCGAACGACTC	CGTCTGTAGCATCAGGGTACTG	60°C	257 bp
Osteocalcin	NM_199173.3	ATGAGAGCCCTCACACTCCTC	GCCGTAGAAGCGCCGATAGGC	62°C	294 bp
Bone sialoprotein	NM_004967	TGACTCATCCGAAGAAAATGGAG	CTGGATTGCAGCTAACCCTGT	60°C	202 bp
Alkaline phosphatase	NM_000478.4	ACGTGGCTAAGAATGTCATC	CTGGTAGGCGATGTCCTTA	53°C	475 bp
Matrix Gla Protein	NM_000900	AGATGGAGAGCTAAAGTCCAAGA	GTAGCGTTCGCAAAGTCTGTA	60°C	102 bp
Osteoblast specific factor 2	NM_006475	TAAGTTTGTTCGTGGTAGCACC	GTGTGGGTCCTTCAGTTTTGATA	60°C	140 kb
BMP2	NM_001200	CCCCCTACATGCTAGACCTGT	CACTCGTTTCTGGTAGTTCTTCC	60°C	150 kb
BMP4	NM_130851	TGGTCTTGAGTATCCTGAGCG	GCTGAGGTTAAAGAGGAAACGA	60°C	130 kb
RANKL	NM_033012.3	TCCCAAGTTCTCATACCCTGA	CATCCAGGAAATACATAACACTCC	56°C	245 bp
Osteoprote-grin	NM_002546.3	CCGGAAACAGTGAATCAACTC	AGGTTAGCATGTCCAATGTG	60°C	313 bp