FAD2-DGAT2 Genes Coexpressed in Endophytic Aspergillus fumigatus Derived from Tung Oilseeds

Table 1

Primer sequences of vfFAD2, vfDGAT2, and 𝐹𝐷𝑀𝑉-2𝐴.

	Primers	Sequences 5^′-3^′
1	vfFAD2 Forward (KpnI)	GGcATGGGTGCTGGTGGC
2	vfFAD2 Reverse (BamHI)	CGggatccAAACTTCTTGTTATACC
3	𝐹𝐷𝑀𝑉-2𝐴   Forward (BamHI)	gatccCAGCTGTTGAATTTTGACCTTCTTAAGCTTG CGGGAGACGTCGAGTCCAACCCCGGGT
4	𝐹𝐷𝑀𝑉-2𝐴  Reverse (XbaI)	gCCCGGGGTTGGACTCGACGTCTCCCGCAAGCTT AAGAAGGTCAAAATTCAACAGCTGAGATC
5	vfDGAT2 Forward (XbaI)	GCtctagaATGGGGATGGTGGAAGTTAAG
6	vfDGAT2 Reverse (PstI)	AActgcagTCAAAAAATTTCAAGTTTAAGG
7	vfFAD2 Forward^′	CGCCGTCACCACTCTAAC
8	vfFAD2 Reverse^′	AGGCCAGCCAAGGGTAAGTGT
9	vfDGAT2 Forward^′	GGGGATGGTGGAAGTTAAGAA
10	vfDGAT2 Reverse^′	AAGCAACCCAATAACGAGGAG

The nucleotides in lowercase letters indicate restriction sites. ATG and TCA in bold indicate the initiation and stop codon, respectively, for the open reading frame of 𝑣𝑓𝐹𝐴𝐷2-𝐹𝑀𝐷𝑉2𝐴-𝑣𝑓𝐷𝐺𝐴𝑇2. Primers 7–10 were used to amplify 𝑣𝑓𝐹𝐴𝐷2 and 𝑣𝑓𝐷𝐺𝐴𝑇2to give PCR products of 150 and 190 bp, respectively.