Vesiculation from Pseudomonas aeruginosa under SOS

Table 1

The WT OMV proteins.

Protein access ID	Protein description	Score	pI	Mass	Predicted functions	Membrane domains
(1) gi∣15595792	OstA precursor [P. aeruginosa PAO1]	366	5.41	104207	Organic solvent tolerance protein	SP, TM
(2) gi∣30525581	ChaPs, heat-shock protein [Piscirickettsia salmonis]	174	4.79	57310	Protein folding, immunogenic protein	n, TM
(3) gi∣15595608	PilJ [P. aeruginosa PAO1]	165	4.65	72484	Cell motility, twitching motility	SP, TM
(4) gi∣15597641	Glycine dehydrogenase [P. aeruginosa PAO1]	133	5.68	103853	Degradation of glycine	n, TM
(5) gi∣254236694	OprC precursor, putative [P. aeruginosa C3719]	117	6.02	79268	Copper transport outer membrane porin	SP, TM
(6) gi∣15599015	SecF [P. aeruginosa PAO1]	115	4.81	33021	Protein translocation	SP, TM
(7) gi∣15599417	Fe(III)-pyochelin OM receptor precursor [P. aeruginosa PAO1]	107	5.86	79943	Siderophore-iron transmembrane transporter activity	SP, TM
(8) gi∣309885	Aspartate transcarbamoylase [P. aeruginosa PAO1]	102	6.17	36521	Pyrimidine biosynthesis	n, n
(9) gi∣83718562	Peptide synthetase-like protein [Burkholderia thailandensis E264]	96	5.91	349402	Peptide synthesis	n, TM
(10) gi∣152986344	YaeT [P. aeruginosa PA7]	94	5.02	88222	OMP assembly complex	SP, TM
(11) gi∣124022818	Phosphate binding protein [Prochlorococcus marinus str. MIT 9303]	90	9.51	18989	ABC transport	SP, TM
(12) gi∣167840097	Phospholipase D [Burkholderia thailandensis MSMB43]	88	6	64431	Lipid catalytic activity	n, TM
(13) gi∣167035883	TonB-dependent copper receptor [P. putida GB-1]	87	5.77	74399	Copper receptor	SP, TM
(14) gi∣28871621	Polyribonucleotide nucleotidyltransferase [P. syringae pv. tomato str. DC3000]	81	5.20	74880	mRNA degradation	n, TM
(15) gi∣15600748	ATP synthase subunit γ [P. aeruginosa PAO1]	73	7.70	31533	ATP production	n,n
(16) gi∣70732441	M48 family peptidase [P. fluorescens Pf-5]	69	7.66	28833	Protein dagradation	SP, TM
(17) gi∣50085971	Acridine efflux pump [Acinetobacter sp. ADP1]	67	6.36	114705	Efflux pump	n, TM
(18) gi∣15596751	Cytochrome oxidase subunit (cbb3-type) [P. aeruginosa PAO1]	66	9.42	53073	Electron transport chain	n, TM
(19) gi∣119713613	SecD [uncultured marine bacterium EB0_39H12]	62	5.63	67763	Protein translocation	n, TM
(20) gi∣7715581	PspA [Streptococcus pneumoniae]	61	4.70	27305	Pneumococcal surface protein A	n, n
(21) gi∣10945103	PapA [P. aeruginosa]	61	4.87	71649	Lipid metabolic process	SP, TM
(22) gi∣121605556	Catalase [Polaromonas naphthalenivorans CJ2]	61	6.26	54212	Hydrogen peroxide catabolic process	n, n
(23) gi∣94495248	Rhizopine catabolism protein [Sphingomonas sp. SKA58]	60	5.91	38825	Oxidation reduction	n, TM
(24) gi∣116328093	Zn-dependent hydrolase [Leptospira borgpetersenii serovar Hardjo-bovis L550]	59	5.67	30997	Hydrolase activity	SP, TM
(25) gi∣149378317	PAS [Marinobacter algicola DG893]	59	5.97	57059	Signal transducer activity, chemotaxis	TM
(26) gi∣119356290	Molybdate ABC transporter, ATPase subunit [Chlorobium phaeobacteroides DSM 266]	59	9.23	38876	ABC transport	n, n
(27) gi∣117619653	Methyl-accepting chemotaxis transducer [P. stutzeri A1501]	59	4.67	58670	Methyl-accepting chemotaxis	SP, TM
(28) gi∣119899764	MCP-domain-containing signal transduction protein [Azoarcus sp. BH72]	59	5.80	53615	Signal transduction	SP, TM
(29) gi∣887858	DcrH [Desulfovibrio vulgaris str. Hildenborough]	59	5.71	104664	Signal transduction	SP, TM
(30) gi∣28869218	Aerotaxis receptor [P. syringae pv. tomato str. DC3000]	59	5.70	56965	Aerotaxis	n, TM
(31) gi∣112004994	Symbionin [Buchnera aphidicola]	58	5.13	57904	Protein folding	n, TM
(32) gi∣15597430	PslD, biofilm proteins [P. aeruginosa PAO1]	58	8.66	27891	polysaccharide transmembrane transport	SP, TM
(33) gi∣154253615	Molybdenum cofactor biosynthesis protein C [Parvibaculum lavamentivorans DS-1]	58	9.64	16871	Cofactor biosynthesis	n, n
(34) gi∣15600235	PilO [P. aeruginosa PAO1]	57	5.04	22805	Cell motility, type 4 fimbrial biogenesis	n, TM
(35) gi∣29830660	Oxidoreductase [Streptomyces avermitilis MA-4680]	57	5.01	33162	Electron transport chain	n, TM
(36) gi∣108803685	Bifunctional homocysteine S-methyltransferase [Rubrobacter xylanophilus DSM 9941]	57	6.33	65183	Amino-acid biosynthesis	n, TM
(37) gi∣15595488	OprE precursor [P. aeruginosa PAO1]	54	8.67	49637	Anaerobically-induced OM porin	SP, TM
(38) gi∣121997032	Glycolate oxidase iron-sulfur subunit [Halorhodospira halophila SL1]	53	8.55	43945	Oxidation reduction	n, n
(39) gi∣161508096	Biotin-acetyl-CoA-carboxylase ligase [Lactobacillus helveticus DPC 4571]	53	9	37120	Protein modification process	n, n
(40) gi∣119773234	GTPase EngB [Shewanella amazonensis SB2B]	52	6.97	24168	Cell division	n, n
(41) gi∣75764772	FtsK [Bacillus thuringiensis serovar israelensis ATCC 35646]	50	4.33	40378	Cell division	SP, TM
(42) gi∣3237312	FimV [P. aeruginosa PAO1]	50	4.33	98113	Cell motility, twitching motility	SP, TM
(43) gi∣145588417	Ferric uptake regulator family protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1]	50	6.29	17176	Repressor of the iron transport operon	n, n
(44) gi∣163846285	Deoxyribose-phosphate aldolase [Chloroflexus aurantiacus J-10-fl]	50	6.08	27440	Deoxyribonucleotide catabolic process	n, n
(45) gi∣107099581	Hypothetical+C448_01000594 [P. aeruginosa PACS2]	267	5.13	101779	Unknown	Unknown
(46) gi∣15598924	Hypothetical PA3729 [P. aeruginosa PAO1]	249	5.18	75836	Unknown	n, TM
(47) gi∣15599685	Hypothetical PA4489 [P. aeruginosa PAO1]	179	5.48	167326	Putative endopeptidase inhibitor activity	SP, TM
(48) gi∣156932378	Hypothetical ESA_00154 [Enterobacter sakazakii ATCC BAA-894]	167	4.83	57278	Protein folding	n, TM
(49) gi∣15596261	Hypothetical PA1064 [P. aeruginosa PAO1]	116	5.38	24157	Unknown	n, TM
(50) gi∣218890269	Hypothetical PLES_15291 [P. aeruginosa LESB58]	97	6.34	28636	Unknown	SP, TM
(51) gi∣15595825	Hypothetical PA0628 [P. aeruginosa PAO1]	92	8.80	35848	Unknown	n, n
(52) gi∣15600425	Hypothetical PA5232 [P. aeruginosa PAO1]	90	9.11	38586	Protein transporter	SP, TM
(53) gi∣15596143	Hypothetical PA0946 [P. aeruginosa PAO1]	86	4.93	36754	Unknown	SP, TM
(54) gi∣107103341	Hypothetical PaerPA_01004410 [P. aeruginosa PACS2]	84	9.02	67631	Unknown	Unknown
(55) gi∣15599691	Hypothetical PA4495 [P. aeruginosa PAO1]	84	5.79	24864	Unknown	SP, TM
(56) gi∣15600607	Hypothetical PA5414 [P. aeruginosa PAO1]	75	5.76	22533	Unknown	SP, TM
(57) gi∣1162960	Protein homologous to HI0366 in Haemophilus influenzae [P. aeruginosa PAO1]	72	6.77	22403	Unknown	SP, TM
(58) gi∣148980343	Hypothetical VSWAT3_23674 [Vibrionales bacterium SWAT-3]	59	4.85	56161	Chemotaxis	SP, TM
(59) gi∣86146473	Hypothetical MED222_12698 [Vibrio sp. MED222]	59	4.76	68870	Signal transducer activity	n, TM
(60) gi∣32266390	Hypothetical HH0891 [Helicobacter hepaticus ATCC 51449]	59	4.99	52013	Signal transducer activity	n, n
(61) gi∣89893845	Hypothetical DSY1099 [Desulfitobacterium hafniense Y51]	59	5.00	54148	Signal transducer activity	n, n
(62) gi∣18309647	Hypothetical CPE0665 [Clostridium perfringens str. 13]	59	4.91	28888	Unknown	Unknown
(63) gi∣160938898	Hypothetical CLOBOL_03792 [Clostridium bolteae ATCC BAA-613]	59	4.79	60828	Chemotaxis	SP, TM
(64) gi∣167772543	Hypothetical ANACOL_03921 [Anaerotruncus colihominis DSM 17241]	59	4.66	71478	Signal transducer activity	SP, TM
(65) gi∣158334828	Hypothetical AM1_1665 [Acaryochloris marina MBIC11017]	59	4.54	28589	Unknown	n, n
(66) gi∣146298741	Hypothetical Fjoh_0980 [Flavobacterium johnsoniae UW101]	58	8.90	168226	Unknown	SP, TM
(67) gi∣126348240	Conserved hypothetical [Streptomyces ambofaciens ATCC 23877]	58	4.68	123551	Unknown	Unknown
(68) gi∣162454210	Hypothetical sce5933 [Sorangium cellulosum So ce 56]	56	10.06	49450	Unknown	SP, TM
(69) gi∣159184340	Hypothetical Atu0493 [Agrobacterium tumefaciens str. C58]	56	8.83	17570	Unknown	Unknown
(70) gi∣120536835	Hypothetical Maqu_4123 [Marinobacter aquaeolei VT8]	54	6.02	36805	Unknown	n, n
(71) gi∣88800650	Hypothetical MED297_05259 [Reinekea sp. MED297]	53	5.05	74044	Unknown	Unknown
(72) gi∣107103648	Hypothetical PaerPA_01004718 [P. aeruginosa PACS2]	52	8.92	52714	Unknown	Unknown
(73) gi∣124385398	Hypothetical BMA10229_A0995 [Burkholderia mallei NCTC 10229]	52	5.66	5192	Unknown	n, n
(74) gi∣94986987	Hypothetical LI0545 [Lawsonia intracellularis PHE/MN1-00]	51	6.43	11768	Unknown	n, n

TM: transmembrane domains; SP: signal peptide; pI: isoelectric point.