Table 1: The WT OMV proteins.

Protein access IDProtein descriptionScorepIMassPredicted functionsMembrane domains

(1) gi∣15595792OstA precursor [P. aeruginosa PAO1]3665.41104207Organic solvent tolerance proteinSP, TM
(2) gi∣30525581ChaPs, heat-shock protein [Piscirickettsia salmonis]1744.7957310Protein folding, immunogenic proteinn, TM
(3) gi∣15595608PilJ [P. aeruginosa PAO1]1654.6572484Cell motility, twitching motilitySP, TM
(4) gi∣15597641Glycine dehydrogenase [P. aeruginosa PAO1]1335.68103853Degradation of glycinen, TM
(5) gi∣254236694OprC precursor, putative [P. aeruginosa C3719]1176.0279268Copper transport outer membrane porinSP, TM
(6) gi∣15599015SecF [P. aeruginosa PAO1]1154.8133021Protein translocationSP, TM
(7) gi∣15599417Fe(III)-pyochelin OM receptor precursor [P. aeruginosa PAO1]1075.8679943Siderophore-iron transmembrane transporter activitySP, TM
(8) gi∣309885Aspartate transcarbamoylase [P. aeruginosa PAO1]1026.1736521Pyrimidine biosynthesisn, n
(9) gi∣83718562Peptide synthetase-like protein [Burkholderia thailandensis E264]965.91349402Peptide synthesisn, TM
(10) gi∣152986344YaeT [P. aeruginosa PA7]945.0288222OMP assembly complexSP, TM
(11) gi∣124022818Phosphate binding protein [Prochlorococcus marinus str. MIT 9303]909.5118989ABC transportSP, TM
(12) gi∣167840097Phospholipase D [Burkholderia thailandensis MSMB43]88664431Lipid catalytic activityn, TM
(13) gi∣167035883TonB-dependent copper receptor [P. putida GB-1]875.7774399Copper receptorSP, TM
(14) gi∣28871621Polyribonucleotide nucleotidyltransferase [P. syringae pv. tomato str. DC3000]815.2074880mRNA degradationn, TM
(15) gi∣15600748ATP synthase subunit γ [P. aeruginosa PAO1]737.7031533ATP productionn,n
(16) gi∣70732441M48 family peptidase [P. fluorescens Pf-5]697.6628833Protein dagradationSP, TM
(17) gi∣50085971Acridine efflux pump [Acinetobacter sp. ADP1]676.36114705Efflux pumpn, TM
(18) gi∣15596751Cytochrome oxidase subunit (cbb3-type) [P. aeruginosa PAO1]669.4253073Electron transport chainn, TM
(19) gi∣119713613SecD [uncultured marine bacterium EB0_39H12]625.6367763Protein translocationn, TM
(20) gi∣7715581PspA [Streptococcus pneumoniae]614.7027305Pneumococcal surface protein An, n
(21) gi∣10945103PapA [P. aeruginosa]614.8771649Lipid metabolic processSP, TM
(22) gi∣121605556Catalase [Polaromonas naphthalenivorans CJ2]616.2654212Hydrogen peroxide catabolic processn, n
(23) gi∣94495248Rhizopine catabolism protein [Sphingomonas sp. SKA58]605.9138825Oxidation reductionn, TM
(24) gi∣116328093Zn-dependent hydrolase [Leptospira borgpetersenii serovar Hardjo-bovis L550]595.6730997Hydrolase activitySP, TM
(25) gi∣149378317PAS [Marinobacter algicola DG893]595.9757059Signal transducer activity, chemotaxisTM
(26) gi∣119356290Molybdate ABC transporter, ATPase subunit [Chlorobium phaeobacteroides DSM 266]599.2338876ABC transportn, n
(27) gi∣117619653Methyl-accepting chemotaxis transducer [P. stutzeri A1501]594.6758670Methyl-accepting chemotaxisSP, TM
(28) gi∣119899764MCP-domain-containing signal transduction protein [Azoarcus sp. BH72]595.8053615Signal transductionSP, TM
(29) gi∣887858DcrH [Desulfovibrio vulgaris str. Hildenborough]595.71104664Signal transductionSP, TM
(30) gi∣28869218Aerotaxis receptor [P. syringae pv. tomato str. DC3000]595.7056965Aerotaxisn, TM
(31) gi∣112004994Symbionin [Buchnera aphidicola]585.1357904Protein foldingn, TM
(32) gi∣15597430PslD, biofilm proteins [P. aeruginosa PAO1]588.6627891polysaccharide transmembrane transportSP, TM
(33) gi∣154253615Molybdenum cofactor biosynthesis protein C [Parvibaculum lavamentivorans DS-1]589.6416871Cofactor biosynthesisn, n
(34) gi∣15600235PilO [P. aeruginosa PAO1]575.0422805Cell motility, type 4 fimbrial biogenesisn, TM
(35) gi∣29830660Oxidoreductase [Streptomyces avermitilis MA-4680]575.0133162Electron transport chainn, TM
(36) gi∣108803685Bifunctional homocysteine S-methyltransferase [Rubrobacter xylanophilus DSM 9941]576.3365183Amino-acid biosynthesisn, TM
(37) gi∣15595488OprE precursor [P. aeruginosa PAO1]548.6749637Anaerobically-induced OM porinSP, TM
(38) gi∣121997032Glycolate oxidase iron-sulfur subunit [Halorhodospira halophila SL1]538.5543945Oxidation reductionn, n
(39) gi∣161508096Biotin-acetyl-CoA-carboxylase ligase [Lactobacillus helveticus DPC 4571]53937120Protein modification processn, n
(40) gi∣119773234GTPase EngB [Shewanella amazonensis SB2B]526.9724168Cell divisionn, n
(41) gi∣75764772FtsK [Bacillus thuringiensis serovar israelensis ATCC 35646]504.3340378Cell divisionSP, TM
(42) gi∣3237312FimV [P. aeruginosa PAO1]504.3398113Cell motility, twitching motilitySP, TM
(43) gi∣145588417Ferric uptake regulator family protein [Polynucleobacter necessarius subsp. asymbioticus QLW-P1DMWA-1]506.2917176Repressor of the iron transport operonn, n
(44) gi∣163846285Deoxyribose-phosphate aldolase [Chloroflexus aurantiacus J-10-fl]506.0827440Deoxyribonucleotide catabolic processn, n
(45) gi∣107099581Hypothetical+C448_01000594 [P. aeruginosa PACS2]2675.13101779UnknownUnknown
(46) gi∣15598924Hypothetical PA3729 [P. aeruginosa PAO1]2495.1875836Unknownn, TM
(47) gi∣15599685Hypothetical PA4489 [P. aeruginosa PAO1]1795.48167326Putative endopeptidase inhibitor activitySP, TM
(48) gi∣156932378Hypothetical ESA_00154 [Enterobacter sakazakii ATCC BAA-894]1674.8357278Protein foldingn, TM
(49) gi∣15596261Hypothetical PA1064 [P. aeruginosa PAO1]1165.3824157Unknownn, TM
(50) gi∣218890269Hypothetical PLES_15291 [P. aeruginosa LESB58]976.3428636UnknownSP, TM
(51) gi∣15595825Hypothetical PA0628 [P. aeruginosa PAO1]928.8035848Unknownn, n
(52) gi∣15600425Hypothetical PA5232 [P. aeruginosa PAO1]909.1138586Protein transporterSP, TM
(53) gi∣15596143Hypothetical PA0946 [P. aeruginosa PAO1]864.9336754UnknownSP, TM
(54) gi∣107103341Hypothetical PaerPA_01004410 [P. aeruginosa PACS2]849.0267631UnknownUnknown
(55) gi∣15599691Hypothetical PA4495 [P. aeruginosa PAO1]845.7924864UnknownSP, TM
(56) gi∣15600607Hypothetical PA5414 [P. aeruginosa PAO1]755.7622533UnknownSP, TM
(57) gi∣1162960Protein homologous to HI0366 in Haemophilus influenzae [P. aeruginosa PAO1]726.7722403UnknownSP, TM
(58) gi∣148980343Hypothetical VSWAT3_23674 [Vibrionales bacterium SWAT-3]594.8556161ChemotaxisSP, TM
(59) gi∣86146473Hypothetical MED222_12698 [Vibrio sp. MED222]594.7668870Signal transducer activityn, TM
(60) gi∣32266390Hypothetical HH0891 [Helicobacter hepaticus ATCC 51449]594.9952013Signal transducer activityn, n
(61) gi∣89893845Hypothetical DSY1099 [Desulfitobacterium hafniense Y51]595.0054148Signal transducer activityn, n
(62) gi∣18309647Hypothetical CPE0665 [Clostridium perfringens str. 13]594.9128888UnknownUnknown
(63) gi∣160938898Hypothetical CLOBOL_03792 [Clostridium bolteae ATCC BAA-613]594.7960828ChemotaxisSP, TM
(64) gi∣167772543Hypothetical ANACOL_03921 [Anaerotruncus colihominis DSM 17241]594.6671478Signal transducer activitySP, TM
(65) gi∣158334828Hypothetical AM1_1665 [Acaryochloris marina MBIC11017]594.5428589Unknownn, n
(66) gi∣146298741Hypothetical Fjoh_0980 [Flavobacterium johnsoniae UW101]588.90168226UnknownSP, TM
(67) gi∣126348240Conserved hypothetical [Streptomyces ambofaciens ATCC 23877]584.68123551UnknownUnknown
(68) gi∣162454210Hypothetical sce5933 [Sorangium cellulosum So ce 56]5610.0649450UnknownSP, TM
(69) gi∣159184340Hypothetical Atu0493 [Agrobacterium tumefaciens str. C58]568.8317570UnknownUnknown
(70) gi∣120536835Hypothetical Maqu_4123 [Marinobacter aquaeolei VT8]546.0236805Unknownn, n
(71) gi∣88800650Hypothetical MED297_05259 [Reinekea sp. MED297]535.0574044UnknownUnknown
(72) gi∣107103648Hypothetical PaerPA_01004718 [P. aeruginosa PACS2]528.9252714UnknownUnknown
(73) gi∣124385398Hypothetical BMA10229_A0995 [Burkholderia mallei NCTC 10229]525.665192Unknownn, n
(74) gi∣94986987Hypothetical LI0545 [Lawsonia intracellularis PHE/MN1-00]516.4311768Unknownn, n

TM: transmembrane domains; SP: signal peptide; pI: isoelectric point.