Microscopic Analysis of Severe Structural Rearrangements of the Plant Endoplasmic Reticulum and Golgi Caused by Overexpression of Poa semilatent virus Movement Protein
Co-expression of non-fused 18 K with the ER and Golgi markers in bombarded epidermal cells of N. benthamiana leaves. (a) ST-YFP. (b)–(d) ST-YFP + 18 K. (e) m-GFP5-ER. (f) and (g) m-GFP5-ER + 18 K. (h) ST-YFP + m-GFP5-ER + 18 K. In (h), GFP signal is shown in the left panel, YFP signal—in the middle panels, and the merged image—in the right panel. All images except (b) and (c) and the insert in (g) are reconstructed by superposition of series of confocal optical sections. Arrowheads in (h) point to round structures of 0.5–1.0 μm in diameter presumably representing Golgi stacks remained unaffected upon the 18 K expression. Scale bar: 20 μm in (a), (b), (f), (g), and (h); 10 μm in (e); 4 μm in (c) and (d); 3 μm in the insert in (g).
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