Microscopic Analysis of Severe Structural Rearrangements of the Plant Endoplasmic Reticulum and Golgi Caused by Overexpression of Poa semilatent virus Movement Protein
Co-expression of 18KIId8 and its fusions with the ER and Golgi markers in bombarded epidermal cells of N. benthamiana leaves. (a)–(c) GFP-18KIId8. (d) ST-YFP + GFP-18KIId8. (e) m-GFP5-ER + YFP-18KIId8. (f) m-GFP5-ER + ST-YFP + 18KIId8. In (d)–(f), GFP signal is shown in the left panels, YFP signal—in the middle panels, and merged images—in the right panels. All images except (c) are reconstructed by superposition of series of confocal optical sections. (c) represents a single optical section in a cell peripheral region. Scale bar: 20 μm in (a), (d)–(f); 10 μm in (b); 4 μm in (c).
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