Research Article

Impact of Environmental Thermal Stimulation on Activation of Hypothalamic Neuronal Nitric Oxide Synthase during the Prenatal Ontogenesis in Muscovy Ducks

Figure 2

Hypothalamic nNOS expression in 20- to 33-days-old Muscovy duck embryos under different temperature treatments (control: constant 37.5°C, warm: 3 h 39°C, cold: 3 h 34°C before start of the experiments). Each column represents mean values ± SD of 100 (100 × 100 μm) rectangle fields from 4–8 brain slices of each experimental group. Investigations were carried out in 80 embryos (see Table 1). Asterisks represent significance at the level of 𝑃 < 0 . 0 0 1 .
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