416936.fig.002
Figure 2: Hypothalamic nNOS expression in 20- to 33-days-old Muscovy duck embryos under different temperature treatments (control: constant 37.5°C, warm: 3 h 39°C, cold: 3 h 34°C before start of the experiments). Each column represents mean values ± SD of 100 (100 × 100 μm) rectangle fields from 4–8 brain slices of each experimental group. Investigations were carried out in 80 embryos (see Table 1). Asterisks represent significance at the level of 𝑃 < 0 . 0 0 1 .